1 | PEB
Product Sizes
5 x 2 ml (4x stock) allows up to 75 isolations of plant material (using 500 ul 1x PEB for 100 mg fresh weight) or 190 isolations of algal material (using 200 ul 1x PEB for cell amounts corresponding to 4-10 ug total chlorophyll)
AS08300-5X2ML
About this Product
- SKU:
- AS08300
- Application:
- Purification
- Extra Details:
- PEB is an extraction buffer for disruption and solubilisation of total protein from plant tissue and algal cells. The use of the anionic detergent LDS together with one of the recommended procedure (combination of sonication and freeze/thaw cycles) has been shown to increase the number of solubilised and non-degraded proteins when compared to other methods of cell disruption (see reference). The estimated hands-on time for the recommended procedure is 20-30 minutes for 1-2 samples. Expected yields will be 1.5-6 µg/µl total protein (recovered from standard procedure) depending on the starting material, e.g. its biological stage, homogenization method used (bead beater vs. sonication).
- Reactivities:
- Other Species
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- Stable at RT for at least 1 month; short-term storage (6 monthss) at 4°C and long term storage (1 year or more) at -20°C.
- Supplier:
- Agrisera
- Type:
- Buffers & General Consumables:Buffers
